RESEALED ERYTHROCYTES

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INTRODUCTION: {1} Now-a-days research is being carried out for the development of drug delivery system with maximum therapeutic effects. Introduction of a probe into the existing delivery system results in the development of a new safe drug delivery system with maximum therapeutic effects and minimal side effects. Erythrocytes otherwise called as red blood cells are being used as carriers and drug-loaded microspheres as well. WHY ARE THESE CALLED RESEALED ERYTHROCYTES,...?{1} Carrier erythrocytes are prepared by collecting the blood samples from the selected organism, separating the RBC from the plasma, entrapping the drug in the erythrocytes and resealing the resultant carrier. Based on the procedure of their preparation they are hence termed as resealed erythrocytes. METHODS OF DRUG LOADING: {1} Several methods have been developed for the preparation of these carrier erythrocytes like physical osmosis based systems (e.g: electrical pulse method) and chemical methods (e.g: chemical perturbation of the erythrocyte membrane) etc. But whatever may be the process employed the drug being sealed should possess certain properties for its successful entrapment. They are: 1.Considerable degree of water solubility. 2.It should not have any physical or chemical interactions with the erythrocyte membrane. 3.It should be resistant against the degradation within erythrocytes. 4.It should possess well-defined pharmacokinetic and pharmacodynamic properties. The methods employed for the preparation of resealed erythrocytes can be classified as follows: 1. Hypo-osmotic lysis method: a. Dilution method b. Dialysis method c. Preswell method d. Isotonic osmotic lysis method 2. Electrical breakdown method 3. Endocytosis method 4. Membrane perturbation method ADVANTAGES OF ERYTHROCYTES AS CARRIERS: {1} 1. Their ability to circulate through out the body. 2. Uniform size and shape of the carrier. 3. Their biodegradability with no generation of toxic products. 4. A wide variety of chemicals can be entrapped. 5. Possibility of ideal zero-order kinetics. 6. Attainment of steady state plasma concentration decreases fluctuations in concentration etc... LIMITATIONS: {1} 1.Limited potential as carrier to non-phagocytic target tissue. 2.Possible clumping of RBC and there by dose dumping may occur. CONCLUSION: {1} Use of resealed erythrocytes has gained massive importance in modern day research. It looks promising for safe delivery of various drugs. However, the concept of resealed erythrocytes needs further optimization to become a routine drug delivery system. RERFERENCES: {1}PHARMA BUZZ, AN ASSEMBLAGE OF PHARMACEUTICAL RESOURSES, A NATIONAL PHARMA MONTHLY MAGAZINE, MAY 2011, VOLUME 6, ISSUE 05. "THIS BLOG DOES NOT CONTAIN ANY PLAGIARISED MATERIAL"

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Siva Mavuduru's picture

hi sir its really interesting and good blog what are the organisms used for the collection of the rbc? and what are the drugs that are delivered through this system?
Siriki Praveen Kumar's picture

hi siva thankyou.... The organisms used for the collection of erythrocytes are those which are to be treated with the loaded erythrocytes..... For example for treatment of human beings human blood should be collected and loaded with the drug of choice,... Examples of drugs dekivered through this system include antineoplastics, antiparasitics, veterinary antiamoebics, vitamins, steroids, antibiotics and cardiovascular drugs etc,,,,.......

Regards,

S. Praveen Kumar.

sirikipraveen11@gmail.com

Pharma warriors

VOLETI VIJAYA KUMAR's picture

What are all the factors to be considered during loading of drugs in RBC? What about the stability aspects? Can you give two marketed formulations?

VIJAYA KUMAR.VOLETI M.Pharmacy

Asst Professor

Raos college of Pharamcy

Nellore

Siriki Praveen Kumar's picture

Factors to be considered resealed erythrocytes as carrier: 1. The shape and size of the RBC to pass easily through the capillaries.... 2. Its specific physico-chemical properties.... 3. Its biocompatibility,,... 4. It should have minimum toxicity nature..... 5. Minimum leaking of drug should occur before it reaches the target site...... 6. Its release pattern should be a controlled one.... 7. Moreover beyond all these properties the carrier system should have high stability during storage,,..

Regards,

S. Praveen Kumar.

sirikipraveen11@gmail.com

Pharma warriors

Sravani kompella's picture

hello praveen, Why should the drug need to be water soluble?can drugs having high o/w ratio be entrapped in erythrocytes? By which mechanism is the drug released from the resealed erythrocytes in side the body?
Siriki Praveen Kumar's picture

Thankyou sravani.... the drug from the resealed erythrocytes can be released by either of the following mechanisms like phagocytosis, diffusion through the membrane of the cell or by using a specific transport system.

Regards,

S. Praveen Kumar.

sirikipraveen11@gmail.com

Pharma warriors

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