PROTEIN STABILITY

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Hello viewers, Its time for the second blog of this month. From the previous blog we have seen that proteins may be folded into 3D form which is a functional state. But this structure may not be attained indefinitely in an aqueous solution. This is due to the fact that proteins are neither chemically or physically stable. Examples of Chemical alterations: #Oxidation of methionyl residues #Hydrolysis reaction on amides and peptide bonds of aspargine and glutamine Physical stability: Physical stability is the difference between the native and denatured states ^Gu Proteins exists in equilibrium between the two states. Most of the proteins denature upon heating and subsequent aggregation of these denatured molecules results in irreversible denaturation. Storage stability of proteins is enhanced by the use of protein stabilizing agents which primarily increases the stability. Use of detergents also minimizes the Irreversible unfolding due to aggregation. Another popular method for enhancing storage stability of proteins is the Lyophilization or freeze dry method. Reference: Pharmaceutical Biotechnology - An Introduction for Pharmacists and Pharmaceutical Scientists, Edited by Daan J A Crommelin and Robert D Sindelar, 2nd Edition, Routledge Taylor and Francis Group, Page no :39-41 THIS BLOG DOES NOT CONTAIN ANY PLAGIARIZED MATERIAL
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Siva Mavuduru's picture

Sir A good and simple blog once again from you
Dr Girija Sankar's picture

Thank you ganesh!

Dr.G.Girija Sankar, Associate Professor Department of Pharmaceutical Biotechnology, College of Pharmaceutical Science, Andhra University.

Ankur sood's picture

nice blog cover important information
Dr Girija Sankar's picture

Thank you!

Dr.G.Girija Sankar, Associate Professor Department of Pharmaceutical Biotechnology, College of Pharmaceutical Science, Andhra University.

G.Sailesh's picture

What are the different factors responsible for causing physical instability of proteins?
Dr Girija Sankar's picture

Physical instability of proteins can be generalized. As you have read in Physical pharmacy, general factors like pH, Temperature, Co solvents, Conductivity, Redox Potential, Concentration of Protein, etc play a vital role. The same employs here. Check out the following link: http://www.lk-processguide.com/proteinstability.php (accessed on July 31st 2011)

Dr.G.Girija Sankar, Associate Professor Department of Pharmaceutical Biotechnology, College of Pharmaceutical Science, Andhra University.

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