Why reverse phase column is preferred over normal phase columns in separation technology

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V.B.S.Aishwarya's picture


Reverse phase columns have a hydrophobic stationary phase which works well for retention of most organic analytes. This also means that water can be used as a mobile phase in conjuntion with less polar solvents such as MeCN and MeOH which can be adjusted at highly controlled rates to improve chromatographic performance. Gradient separations in normal phase are much more complicated because of UV cutoff variation as well as differences in compressability of common hydrophobic solvents, which would have an effect on flow rate. Reverse phase chromatography also has the advantage of being able to use pH selectivity to improve separations.






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