Why now analysis ppl only do methord validation of drugs by RpHPLC now?

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manjusha's picture


In Reverse phase chromatography mobile phase is more polar(aqueous) compared to stationary phase...hydrophobic molecules in the polar mobile phase tend to adsorb to the hydrophobic stationary phase, and hydrophilic molecules in the mobile phase will pass through the column and are eluted first...Hydrophobic molecules can be eluted from the column by decreasing the polarity of the mobile phase using an organic (non-polar) solvent,.Sofor  better selectivity, wider range of pH, better elution and for good resolution RP-column siuts better and occurance of problems is also less...so RP-columns are preffered.......

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