WHAT IS THE COMPOSITION OF DRAGENDORF'S REAGENT AND WHAT IS ITS USE?

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Hardik Soni's picture

Answer: 
Dear Uma, Composition of Dragendorff reagent: Solution (a): Dissolve 0.85g basic bismuth nitrate in 10ml glacial acetic acid and 40 ml water under heating. If necessary, filter. Solution (b): Dissolve 8 g potassium iodide in 30 ml water. Stock solution: (a) + (b) are mixed 1:1 Spray Reagent: 1 ml stock solution is mixed with 2 ml glacial acetic acid an d 10 ml water. Use: Detection of alkaloids, heterocyclic nitrogen compounds. Ref.: Plant Drug Analysis, H.Wagner, Second Edition; P.No. 360 With Regards,

Leader - Ayurpreachers Hardik K. Soni M.Pharm (Ayu.)-Pharmacology R&D, Executive Vasu Research Centre www.vasuresearch.com

VOLETI VIJAYA KUMAR's picture

Answer: 
Solution A: Bismuth nitrate (0.17g) in AcOH (2mL) and H2O (8mL) Solution B: KI (4g) in AcOH (10mL) and H2O (20mL) Mix Solns. A and B and dilute to 100mL with H2O. Dragendorff's reagent is usually used to visualize alkaloids that have been developed using TLC. In forensics it is frequently used for cocaine and opiate detection. Read more: http://wiki.answers.com/Q/How_do_you_prepare_Dragendorff's_reagent#ixzz1BxlQCN1z

VIJAYA KUMAR.VOLETI M.Pharmacy

Asst Professor

Raos college of Pharamcy

Nellore

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