what is the role of linker and adapter molecules in RDNA technique?

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Dr Girija Sankar's picture

These are small nucleotide molecules with few base pairs that are used to ligate the gene of interest to the vector DNA. Linkers are short double stranded DNA fragments that contain restriction endonucleases cleavage sites. These can be ligated to blunt ends of any DNA fragment and cut with a specific R.E. to generate DNA fragments with cohesive ends. Adapters are chemically synthesized DNA molecules with preformed cohesive ends. They are required when the target site for the RE used is present within the DNA sequence to be cloned. Ref: Genomics and Genetic Engineering by Prathik Satya pg 70

Dr.G.Girija Sankar, Associate Professor Department of Pharmaceutical Biotechnology, College of Pharmaceutical Science, Andhra University.

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